Centre de Formation et d’Exploration Fonctionnelle PRIMates (CE2F-PRIM)

The CE2F-PRIM (Centre de Formation et d'Exploration Fonctionnelle sur modèle PRIMates non humains) (UMS 3537-CNRS) is a unique structure in France and Europe for its functional exploration work in non-human primates (NHP), notably in neuroscience, and for its training activities in the use of animals for scientific purposes.

The centre offers accommodation capacities for non-human primates (Macaca mulatta, Macaca fascicularis, Saimiri sciureus) in outdoor parks or indoor housing, as well as various rodent species, for basic and clinical neuroscience experiments. The centre has A1/A2 housing areas and several ultra-modern surgical platforms dedicated to animal research in NHP and rodents.

Among its scientific and functional objectives, the CE2F-PRIM wishes to set up a structure for the production of neurological pathologies animal models using conventional and molecular approaches, in response to the limitations of mouse models. In close collaboration with the Institut de Neurosciences de la Timone (UMR7289), but also with the Station of Primatology (SdP) in Rousset, CE2F-PRIM is setting up a complete chain for the use of the NHP model in biomedical research, in response to the limitations of mouse models, ranging from resource management to multimodal functional exploration (electrophysiology, multi-scale in vivo imaging). The Centre supports basic research, clinical or private (Biotechnologies, CRO...).

With more than 30 weeks of training per year, including all regulatory training, thematic trainings on different animal models and on surgical models, CE2F-PRIM is one of the most important animal regulatory training centres in Europe.

CE2F-PRIM participates with the SdP to the activities of EUPRIM-Net II Consortium. This network gathers nine European primatology centers whose aim is to combine their extensive biological and biomedical research activities, in-depth knowledge, infrastructure resources and solid experience in hosting primates.

The CE2F-PRIM was created to fulfil 3 main missions. The first objective is to support neuroscience research within INT (Institut de Neurosciences de la Timone) and other laboratoriesin Marseille, particularly for the production of animal models. The second mission is the training of research staff. Its third mission is to provide services (housing, follow-up of biological protocols, experimental surgery) for biomedical R&D of private partners in different fields: neurosciences, pharmacology and immunology.

Support for research

In conjunction with other INT technological platforms, CE2F-PRIM provides access to functional in vivo imaging tools, particularly to conduct experimental protocols. CE2F-PRIM upgrades experimental devices used for animals to improve functional and behavioural exploration devices while improving animal welfare.

  • INT support activities mostly focus on functional exploration in non-human primates:
    ◊ Neurosurgical operations
    ◊ Invasive and non-invasive techniques to decode brain activity and its relationship to behaviour
    ◊ Mass electrophysiology
    ◊ Molecular imaging (PET/SPECT), microscopic (confocal, biphotonic), mesoscopic (optical), macroscopic (3T MRI)
    ◊ Recording studies of motor behaviour and perceptual function disturbances

The CE2F-PRIM ensures all the essential steps to work with animals in optimal conditions of safety, animal welfare, veterinary care and ethics. Different animal models are available (rats, mice, non-human primates).

Trainings

In terms of training, CE2F-PRIM is a national reference center for CNRS et l’INSERM.

  • Each year, CE2F-PRIM organizes more than 30 weeks of regulatory training at all levels (Animal Experimentation "operator" and "designer" levels, Surgery, Zootechnics) for the use of animals for scientific purposes (rodents, non-human primates, pigs, aquatic vertebrates).
    These regulatory training courses are organized in Marseille, but also in Paris area, Bordeaux, Strasbourg, and are intended for research staff: animal technicians, researchers and students.
  • CE2F-PRIM also hosts specialized trainings (electrophysiology, experimental ophthalmology,...) in partnership with the Marseille Faculty of Medicine and private partners.

Value creation

  • Services for biotechnology companies and academic units wishing to conduct their R&D projects in non-human primates or rodents.
  • Support to academic research teams through its surgical platform and accommodation structures

CE2F-PRIM organizes more than 30 weeks of training sessions for scientific and technical staff, either academic or private.

CE2F-RPIM organizes regulatory training in animal experimentation, conform to the requirements of the new European Directive 2010/63 (former levels I and II, and surgery).

The objective is to acquire the mandatory qualification to practice animal experimentation in accordance with the transposition of the new European Directive 2010/63.

CE2F-PRIM also organizes specific trainings in primaology and neurosciences.

Regulatory trainings for development of animal experimental procedures (former level 1)

7-day training programme (5 days for rodent model training with e-learning sessions) for researchers and research engineers with direct scientific responsibility for animal experiments.
The teaching covers animal experimentation regulations and legislation (national regulations and new European directive) as well as experimental procedure design, mutagenesis, ethical principles and applied ethics, the 3Rs rule, alternative methods, basic knowledge of model animal biology, zootechnics, animal health status, animal pain and stress, pathologies, anaesthesia techniques, euthanasia, autopsy,...

Developing experimental procedures on rodents

  • CFE trainings "CNRS formation entreprises": 7 days
    ▪ 2 sessions in paris and outskirts in 2018: May 28 -June 5, November 19-27
    Description
  • Trainings exclusively limited to CNRS employees or non-CNRS employees working in a CNRS unit: 5 days + e-learning
    ▪ 4 sessions in Paris in 2018: March 12-16 with e-learning from 29/01/18 to 23/02/18, April 16-20 with e-learning from 05/02/18 to 30/03/18, September 3-7 with e-larning from 02/07/18 to 24/08/18, October 22-26 with e-learning from 10/09/18 to 08/10/18
    ▪ 3 sessions in Marseille in 2018: March 26-30 with e-learning from 12/02/18 to 09/03/18, October 8-12 with e-learning from 02/07/18 to 21/09/18, December 3-7 with e-learning from 22/10/18 to 16/11/18
    ▪ 1 session in Bordeaux in 2018:
    June 26 - July 5 (no e-learning)

    Contact

  • Developing experimental procedures on rodents in ENGLISH organized by CE2F-PRIM and CELPHEDIA
    Trainings exclusively limited to CNRS employees or non-CNRS employees working in a CNRS unit: 7 days
    ▪ 1 session in Marseille in 2018: June 28 - July 6
    ▪ 1 session in Strasbourg in 2018:
    September 24 - October 2

    Contact

Developing experimental procedures on non human primates

  • This regulatory training is intended for people designing experimental procedures and projects on non-human primate (NHP) models.
    This teaching also discusses the relevance of using the NHP model for scientific purposes:
    - Understand in which research themes the NHP model remains unavoidable and why?
    - Discover and better understand the possibilities of medical imaging applied to the NHP model
    - Demonstrate the importance of experimental models other than the NHP model
    - Reuse of animals in research
    Trainings exclusively limited to CNRS employees or non-CNRS employees working in a CNRS unit: 7 days
    ▪ Session in Marseille in 2018: March 15-23

    Contact

Developing experimental procedures on aquatic vertebrates

  • This regulatory training is intended for people designing experimental procedures and projects on aquatic vertebrates models. Multi-site CNRS training set up thanks to the collaboration of three CELPHEDIA partner centers: TEFOR-AMAGEN, CRB Xenopus and CE2F-PRIM.
    Trainings exclusively limited to CNRS employees or non-CNRS employees working in a CNRS unit: 7 days
    ▪ 2 sessions in Marseille ien 2018: April 5-13, September 13-21

    Contact
    CFE trainings "CNRS formation entreprises": 7 days
    ▪ Sessions in Marseille in 2019: June 17-25, October 21-29
    Description

Regulatory trainings for application of animal experimental procedures (former level 2)

5-day training programme (some of which includes e-learning sessions) for staff directly involved in animal experiments.
The teaching covers animal experimentation regulations and legislation as well as the animal (ethics, 3Rs rule, design of experimental procedures, zootechnical procedures, housing, genetics, basic knowledge of rodent biology, small animal imaging, pathologies, anaesthesia, analgesia and euthanasia, use of appropriate limit points, recognition of signs of distress and pain, hygiene and health control, transport).

Applying experimental procedures on rodents

  • CFE trainings "CNRS formation entreprises": 5 days
    ▪ 4 sessions in Gif-sur-Yvette in 2018: April 9-13, June 4-8, October 1-5, November 5-9
    Description
    ▪ 1 session in Marseille in 2018: September 24-28
    Description

  • Trainings exclusively limited to CNRS employees or non-CNRS employees working in a CNRS unit: 5 days + e-learning
    ▪ 2 sessions in Marseille in 2018: February 12-16 with e-learning from 02/01/18 to 26/01/18, November 26-30 with e-learning from 15/10/18 to 09/11/18
    Contact

Regulatory trainings in surgery

Prior attendance to regulatory trainings for staff either developing or applying experimental procedures is required.
4-day training programme for persons requiring authorisation to perform surgical procedures on animals, in accordance with the transposition of the new European Directive 2010/63/EU.
The teaching covers surgical techniques and preparatory and post-operative care: asepsis, preparation of animals and staff, organization of the premises, choice and maintenance of equipment, anaesthesia, analgesia monitoring, postoperative follow-up, limits in surgery, infection prevention, microsurgical techniques.

  • CFE trainings "CNRS formation entreprises": 4 days
    ▪ 3 sessions in Marseille in 2018: May 15-18, October 16-19, October 22-25
    Description

  • Trainings exclusively limited to CNRS employees or non-CNRS employees working in a CNRS unit: 4 days
    ▪ 5 sessions in Marseille in 2018: February 5-8, April 22-25, June 4-7, October 1-4, November 5-8
    Contact

Trainings in neurosciences

  • Surgery training on rodent model
    Training organized in collaboration with NSRepair company
    This training covers regulatory practices in surgery and ethics, preparation of an intervention (instruments, animals) and surgical approaches to the brain, cerebrovascular system, spinal cord, peripheral nervous system.
    Applications to models of stroke, ischemia, spinal cord injury or peripheral nerve injury.
    ▪ Session in Marseille: next dates not yet defined
  • European Surgical Training for the Utah Electrode Array
    Training on multi-électrode implants organized in collaboration with the US company Blackrock Microsystems.
    The purpose of this workshop is to familiarize participants with the skills required for effective surgical implantation of Utah Array™ in non-human primates.
    ▪ Session in Marseille: next dates not yet defined

Trainings for zootechnicians

  • Participation to the Network Days of the CNRS Zootechnicians (ReZooTech)
    The programme focuses on regulatory developments with the new EU Directive 2010/63/EU and its implementation.
    Trainings exclusively limited to CNRS zootechnicians
    Next session: date and place to be defined

Other trainings

Modules from ½ or 1 day of continuing practical training (sampling methods, anesthesia,...)

Neurosciences

  • Implementation of a structure for the production of neurological pathologies animal models using conventional and molecular approaches, in response to the limitations of mouse models.
  • Functional exploration in neurosurgery on non-human primate and rodent models, to decode brain activity and its links with behaviour
  • Production of PNH models of neurological and psychiatric pathologies

Imaging

  • Development of a biphotonic microscopy system in non-human primates
  • Development of anatomical and functional imaging by 3T MRI on the non-human primate model

CE2F-PRIM logoMarseille

CE2F-PRIM logoNon-human primates, rodents

CE2F-PRIM logodata not available

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